碱性蛋白质毛细管电泳分离研究

碱性蛋白质毛细管电泳分离研究任吉存，邓延倬，程介克（武汉大学化学系分析测试科学系，武汉，４３００７２）关键词毛细管电泳，碱性蛋白质，吸附，精氨酸，赖氨酸蛋白质的吸附作用严重影响了毛细管电泳分离蛋白质的重现性［１］．为此，人们寻找各种途径来克服蛋白质的...

Capillary Zone Electrophoresis Separation of Basic Proteins Using Arginine and Lysine as Buffer Additives

The separation of basic proteins by capillary electrophoresis was plagued by the adsorption of the basic proteins onto the capillary inner surface,Atnethod is presented to solve this problem by using arginine and lysine as buffer additives,It was found that in the neutral or slightly alkaline solution, arginine and lysine can be adsorbed onto the inner surface of capillary to reduce the electroosmotic flow and the adsorption of the proteins efficiently, which results in improvement of resolution efficiency and reproducibility of the migration time and peak height of basic proteins,The experimental results show that arginine and lysine are the efficient buffer additives used in separation of basic proteins.