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Development of a Sensitive Bioluminogenic Probe for Imaging Highly Reactive Oxygen Species in Living Rats |
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| Authors: | Dr Mako Kamiya Prof Eiji Kobayashi Dr Toru Komatsu Dr Tasuku Ueno Dr Takuya Terai Dr Kenjiro Hanaoka Prof Tetsuo Nagano Prof Yasuteru Urano |
| Institution: | 1. Graduate School of Medicine, The University of Tokyo, 7‐3‐1 Hongo Bunkyo‐ku, Tokyo, 113‐0033 (Japan);2. PRESTO (Japan) Science and Technology Agency, 4‐1‐8 Honcho, Kawaguchi, Saitama, 332‐0012 (Japan);3. Department of Organ Fabrication, Keio University School of Medicine, 35 Shinanomachi, Shinjuku‐ku, Tokyo, 160‐8582 (Japan);4. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7‐3‐1 Hongo Bunkyo‐ku, Tokyo, 113‐0033 (Japan);5. Open Innovation Center for Drug Discovery, The University of Tokyo, 7‐3‐1 Hongo Bunkyo‐ku, Tokyo, 113‐0033 (Japan);6. AMED CREST (Japan), Agency for Medical Research and Development, 1‐7‐1 Otemachi, Chiyoda‐ku, Tokyo, 100‐0004 (Japan) |
| Abstract: | A sensitive bioluminogenic probe for highly reactive oxygen species (hROS), SO3H‐APL, was developed based on the concept of dual control of bioluminescence emission by means of bioluminescent enzyme‐induced electron transfer (BioLeT) and modulation of cell‐membrane permeability. This probe enables non‐invasive visualization of physiologically relevant amounts of hROS generated deep inside the body of living rats for the first time. It is expected to serve as a practical analytical tool for investigating a wide range of biological functions of hROS in vivo. The design concept should be applicable to other in vivo bioluminogenic probes. |
| Keywords: | electron transfer imaging agents luminescence membrane permeability reactive oxygen species |