A Single Biosensor for Evaluating the Levels of Copper Ion and L‐Cysteine in a Live Rat Brain with Alzheimer's Disease |
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| Authors: | Dr Yongping Luo Dr Limin Zhang Dr Wei Liu Dr Yanyan Yu Prof Yang Tian |
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| Institution: | 1. Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, North Zhongshan Road 3663, Shanghai 200062 (P.R. China);2. Xinyu Institute of New Energy, Xinyu University, Sunshine Avenue 2666, Xinyu 338004 (China);3. Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Department of Pharmaceutical Analysis, Xuzhou Medical College, Xuzhou 221004 (China) |
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| Abstract: | Copper ion (Cu2+) and L ‐cysteine (CySH) are closely correlated with physiological and pathological events of Alzheimer’s Disease (AD), however the detailed mechanism is still unclear, mainly owing to a lack of accurate analytical methods in live brains. Herein, we report a single biosensor for electrochemical ratiometric detection of Cu2+ and CySH in live rat brains with AD. N,N‐di‐(2‐picoly)ethylenediamine (DPEA) is first synthesized for specific recognition of Cu2+ to form a DPEA–Cu2+ complex. This complex shows high selectivity for CySH owing to the release of Cu2+ from the complex through CySH binding to Cu2+ center. In parallel, 5′‐MB‐GGCGCGATTTTTTTTTTTTT‐SH‐3′ (HS‐DNA‐MB, MB=Methylene Blue) is designed as an inner‐reference for providing a built‐in correction to improve the accuracy. As a result, combined with the amplified effect of Au nanoleaves, our single ratiometric biosensor can be successfully applied in real‐time detection of Cu2+ and CySH in the live rat brains with AD. To our knowledge, this is the first report on the accurate concentrations of Cu2+ and CySH in live rat brains with AD. |
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| Keywords: | Alzheimer’ s disease biosensors brain chemistry copper ions L‐cysteine |
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