An Efficient Labelling Approach to Harness Backbone and Side‐Chain Protons in 1H‐Detected Solid‐State NMR Spectroscopy |
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| Authors: | Deni Mance Dr. Tessa Sinnige Mohammed Kaplan Siddarth Narasimhan Mark Daniëls Dr. Klaartje Houben Prof. Marc Baldus Dr. Markus Weingarth |
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| Affiliation: | 1. NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht (The Netherlands);2. Present address: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW (UK) |
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| Abstract: | 1H‐detection can greatly improve spectral sensitivity in biological solid‐state NMR (ssNMR), thus allowing the study of larger and more complex proteins. However, the general requirement to perdeuterate proteins critically curtails the potential of 1H‐detection by the loss of aliphatic side‐chain protons, which are important probes for protein structure and function. Introduced herein is a labelling scheme for 1H‐detected ssNMR, and it gives high quality spectra for both side‐chain and backbone protons, and allows quantitative assignments and aids in probing interresidual contacts. Excellent 1H resolution in membrane proteins is obtained, the topology and dynamics of an ion channel were studied. This labelling scheme will open new avenues for the study of challenging proteins by ssNMR. |
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| Keywords: | ion channels membrane proteins proton detection side‐chain protons solid‐state NMR spectroscopy |
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