The cis‐Diammineplatinum(II) Complex of Curcumin: A Dual Action DNA Crosslinking and Photochemotherapeutic Agent |
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| Authors: | Koushambi Mitra Srishti Gautam Prof Paturu Kondaiah Prof Akhil R Chakravarty |
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| Institution: | 1. Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore 560012, Karnataka (India);2. Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, Karnataka (India) |
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| Abstract: | Pt(cur)(NH3)2](NO3) ( 1 ), a curcumin‐bound cis‐diammineplatinum(II) complex, nicknamed Platicur, as a novel photoactivated chemotherapeutic agent releases photoactive curcumin and an active platinum(II) species upon irradiation with visible light. The hydrolytic instability of free curcumin reduces upon binding to platinum(II). Interactions of 1 with 5′‐GMP and ct‐DNA indicated formation of platinum‐bound DNA adducts upon exposure to visible light (λ=400–700 nm). It showed apoptotic photocytotoxicity in cancer cells (IC50≈15 μM ), thus forming ?OH, while remaining passive in the darkness (IC50>200 μM ). A comet assay and platinum estimation suggest Pt–DNA crosslink formation. The fluorescence microscopic images showed cytosolic localization of curcumin, thus implying possibility of dual action as a chemo‐ and phototherapeutic agent. |
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| Keywords: | antitumor agents cell imaging curcumin DNA platinum |
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