The impact of extra-column band broadening on the chromatographic efficiency of 5 cm long narrow-bore very efficient columns |
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| Authors: | Fekete Szabolcs Fekete Jeno |
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| Institution: | Budapest University of Technology and Economics, Department of Inorganic and Analytical Chemistry, Szt. Gellért tér 4, 1111 Budapest, Hungary. fekete.szabolcs1@chello.hu |
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| Abstract: | Small columns packed with core-shell and sub-2 μm totally porous particles and monolith columns are very popular to conduct fast and efficient chromatographic separations. In order to carry out fast separations, short (2-5 cm) and narrow-bore (2-2.1 mm) columns are used to decrease the analyte retention volume. Beside the column efficiency, another significant issue is the extra-column band-spreading. The extra-column dispersion of a given LC system can dramatically decrease the performance of a small very efficient column. The aim of this study was to compare the extra-column peak variance contribution of several commercially available LC systems. The efficiency loss of three different type 5 cm long narrow bore, very efficient columns (monolith, sub-2 μm fully porous and sub-2 μm core-shell packing) as a function of extra-column peak variance, and as a function of flow rate and also kinetic plots (analysis time versus apparent column efficiency) are presented. |
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| Keywords: | Column efficiency Extra-column band-broadening Core–shell particles Sub-2 μm particles Monolithic columns UHPLC |
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