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Geranylgeraniol formation in Croton stellatopilosus proceeds via successive monodephosphorylations of geranylgeranyl diphosphate |
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| Authors: | Natsajee Nualkaew Toni M Kutchan |
| Institution: | a Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand b Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany c Biocenter-Pharmacy, University of Halle, Weinbergweg 22, 06120 Halle, Germany |
| Abstract: | The process of catalytic dephosphorylation of geranylgeranyl diphosphate (GGPP) to give geranylgeraniol (GGOH) in Croton stellatopilosus leaves was examined by in vivo chloroplast feedings with 1-3H]GGPP and 1-3H]GGMP and in vitro enzyme-catalyzed reactions. The results strongly suggest that the formation of GGOH from GGPP proceeds in the chloroplasts via two successive monodephosphorylation reactions. Hence, we name the enzyme geranylgeranyl diphosphate phosphatase rather than geranylgeranyl diphosphatase based on its catalytic mechanism. |
| Keywords: | Croton stellatopilosus Euphorbiaceae Terpenoid biosynthesis Chloroplast Geranylgeranyl diphosphate phosphatase Geranylgeraniol |
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