New approach to the synthesis of oligodeoxyribonucleotides modified with phosphorothioates of predetermined sense of P-chirality |
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Authors: | Barbara Nawrot Beata R?bowska Katarzyna Cie?lińska Wojciech J Stec |
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Institution: | Centre of Molecular and Macromolecular Studies of Polish Academy of Sciences, Department of Bioorganic Chemistry, Sienkiewicza 112, 90-363 ?ód?, Poland |
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Abstract: | Appropriately protected, diastereomerically pure dinucleoside phosphorothioates, obtained by the stereocontrolled oxathiaphospholane method, were S-alkylated with 2-nitrobenzyl bromide and then converted into their 3′-O-phosphoramidites. The corresponding S-protected dinucleotide building blocks were successfully used for the synthesis of oligonucleotides containing P-stereodefined phosphorothioate bonds at preselected positions. |
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Keywords: | P-Chiral dinucleoside phosphorothioate Oligonucleotide phosphorothioate PS-DNA S-Protection Oligonucleotide synthesis |
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