泥蚶卵黄原蛋白基因的克隆及其在Pb胁迫下的表达特征

1.宁波大学 海洋学院, 浙江 宁波 315211; 2.浙江万里学院 浙江省水产种质资源高效利用重点实验室, 浙江 宁波 315100

Molecular Cloning and Expression of Pattern Features under Pb Stress for Vitellogenin from Tegillarca granose

Using the SMART RACE method, Vitellogenin(Vg) part cDNA sequence is obtained. The fragment cDNA of Vg gene is 1411bp, which consists of an open reading frame of 1362bp encoding a polypeptide of 454 amino acids. Homology alignment results reveal that the similarity of deduced amino acid sequence with other bivalve shellfish is higher than 49%. The results of tissue-specific expression by real time PCR show that Vg of Tegillarca granosa in breeding period is mainly expressed in ovarian and digestive gland. After challenged by 1, 3, 6, 9μg?L-1 concentration of Pb for 28 days, the results of relative expression in digestive gland of Tegillarca granosa in non-breeding period of Vg mRNA is obtained showing that the first three groups are first slightly reduced followed by a significant increase. Throughout the experiment, Vg mRNA is used as the induced expression and the expression quantity is obtained after the first rise followed by decrease under stress of the concentrations of 9μg?L-1 Pb. Vg expression reaches highest at 21d under Pb stress (P<0.01). In addition, Pb induces Vg expression with the relationship between dose-effect and time-effect. It is speculated that the antioxidant functions play a role in the oxidative damage caused by Pb stress.