Two-dimensional ordered beta-sheet lipopeptide monolayers |
| |
Authors: | Cavalli Silvia Handgraaf Jan-Willem Tellers Emily E Popescu Daniela C Overhand Mark Kjaer Kristian Vaiser Vladimir Sommerdijk Nico A J M Rapaport Hanna Kros Alexander |
| |
Institution: | Contribution from the Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands. |
| |
Abstract: | A series of amphiphilic lipopeptides, ALPs, consisting of an alternating hydrophilic and hydrophobic amino acid residue sequence coupled to a phospholipid tail, was designed to form supramolecular assemblies composed of beta-sheet monolayers decorated by lipid tails at the air-water interface. A straightforward synthetic approach based on solid-phase synthesis, followed by an efficient purification protocol was used to prepare the lipid-peptide conjugates. Structural insight into the organization of monolayers was provided by surface pressure versus area isotherms, circular dichroism, Fourier transform infrared spectroscopy, and Brewster angle microscopy. In situ grazing-incidence X-ray diffraction (GIXD) revealed that lipopeptides six to eight amino acids in length form a new type of 2D self-organized monolayers that exhibit beta-sheet ribbons segregated by lipid tails. The conclusions drawn from the experimental findings were supported by a representative model based on molecular dynamics simulations of amphiphilic lipopeptides at the vacuum-water interface. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|