Anacardium occidentale Bark Lectin: Purification, Immobilization as an Affinity Model and Influence in the Uptake of Technetium-99M by Rat Adipocytes |
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Authors: | Maria Inês Sucupira Maciel Maria do Socorro de Mendon?a Cavalcanti Thiago Henrique Napole?o Patrícia Maria Guedes Paiva Maria Teresa Jansem de Almeida Catanho Luana Cassandra Breitenbach Barroso Coelho |
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Institution: | 1. Departamento de Ci??ncias Dom??sticas, Alimentos Nutri??o e Sa??de, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil 2. Instituto de Ci??ncias Biol??gicas, Universidade de Pernambuco, Recife, Pernambuco, Brazil 3. Departamento de Bioqu??mica, Centro de Ci??ncias Biol??gicas, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-420, Brazil 4. Departamento de Biof??sica e Radiobiologia, Centro de Ci??ncias Biol??gicas, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil
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Abstract: | Lectins, proteins that recognize carbohydrates, have been immobilized on inert supports and used in the screening or purification of glycoproteins. Anacardium occidentale bark infusion has been used as a hypoglycemic agent in Brazil. The toxicity of natural products may be evaluated determining their capability to alter the biodistribution of technetium-99M (99mTc). This work reports the isolation and characterization of a lectin from A. occidentale bark (AnocBL), its evaluation as an affinity support for glycoprotein isolation and lectin effect on the uptake of 99mTc by rat adipocytes. AnocBL was isolated from 80?% ammonium sulphate supernatant by affinity chromatography on fetuin?Cagarose. SDS?CPAGE showed a single protein band of 47?kDa. The monossacharide l-arabinose and the glycoproteins fetuin, asialofetuin, ovomucoid, casein, thyroglobulin, peroxidase, fetal bovine serum and IgG inhibited the activity. The lectin activity was stable until 70?°C and at a pH range of 3.0?C7.5. AnocBL?CSepharose column bound fetuin indicating that the lectin matrix may be used to obtain glycoconjugates of biotechnological interest. In vitro assay revealed that glucose and insulin increase 99mTc uptake by rat adipocytes. AnocBL decreases 99mTc uptake, and this effect was not detected in the presence of glucose. Fetuin inhibited AnocBL effect in all insulin concentrations. |
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