Production of Mixed-Linkage Beta-Oligosaccharides from Lichenan Using Immobilized Bacillus licheniformis UEB CF Lichenase |
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| Authors: | Fatma Chaari Monia Blibech Fatma Bhiri Sameh Maktouf Raoudha Ellouz-Ghorbel Semia Ellouz-Chaabouni |
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| Institution: | 1. Unit?? enzymes et bioconversion, Biology Department, Sfax National School of Engineers, Sfax University, B.P. 1173, 3038, Sfax Cedex, Tunisia
2. Unit?? enzymes et bioconversion and Unit?? de service commun bior??acteur coupl?? ?? un ultrafiltre, Biology Department, Sfax National School of Engineers, B.P. 1173, 3038, Sfax Cedex, Tunisia
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| Abstract: | Lichenase from Bacillus licheniformis UEB CF was immobilized on Amberlite IR120 H. The immobilization yield and lichenase activity were 87 and 92.81?% of initial activity, respectively. The immobilized enzyme exhibited a shift in the optimal pH from 5.0 to 3.0, but the activity optimal temperature was not affected. The immobilized enzyme showed a residual activity of 50?% after five uses. It also exhibited high storage stability and retained 50?% of its initial activity after 120?days at 4?°C. The main hydrolysis products yielded from lichenan were trisaccharide and tetrasaccharide. The resulting mixed-linkage beta-oligosaccharides could be used as a special nutriment for lactic bacteria. |
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