A Fast and Efficient Ultrasound-Assisted Extraction of Tocopherols in Cow Milk Followed by HPLC Determination |
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| Authors: | Archimede Rotondo Giovanna Loredana La Torre Teresa Gervasi Giacomo di Matteo Mattia Spano Cinzia Ingallina Andrea Salvo |
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| Institution: | 1.Department of Biomedical and Dental Science and Morpho-Functional Imaging (BIOMORF), University of Messina, Polo Universitario Annunziata, 98168 Messina, Italy; (A.R.); (T.G.);2.Department of Drug Chemistry and Technologies, University of Rome “La Sapienza”, P.le Aldo Moro, 5, 00185 Roma, Italy; (G.d.M.); (M.S.); (C.I.) |
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| Abstract: | A fast HPLC method with fluorescence detector (FD) was developed for the determination of three tocopherols (TOCs) in milk samples from Modicana cattle breed. The ultrasound-assisted procedure was optimized for the extraction of TOCs prior to HPLC/FD analysis, reducing sample preparation time and allowing a fast quantification of α-tocopherol, δ-tocopherol and γ tocopherol. The optimized ultrasonic extraction combines an efficient and simple saponification at room temperature and a rapid HPLC quantification of TOCs in milk. The precision of the full analytical procedure was satisfactory and the recoveries at three spiked levels were between 95.3% and 87.8%. The linear correlations were evaluated (R2 > 0.99) and the relative standard deviation (RSD) values for intra-day and inter-day tests at three spiked levels were below 1% for the retention time and below 5.20% for the area at low level spiking. The proposed procedure, reducing the experimental complexity, allowed accurate extraction and detection of three TOCs in milk samples from Modicana cattle breed. |
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| Keywords: | tocopherol vitamin E milk food analysis HPLC/FD analysis |
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