A Study of the Interaction,Morphology, and Structure in Trypsin-Epigallocatechin-3-Gallate Complexes |
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| Authors: | Jiayin Liu Hossein Ghanizadeh Xinmao Li Zhengyuan Han Youwen Qiu Yao Zhang Xiuling Chen Aoxue Wang |
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| Affiliation: | 1.College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; (J.L.); (X.L.); (Z.H.); (X.C.);2.College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China;3.School of Agriculture and Environment, Massey University, 4410 Palmerston North, New Zealand;4.College of Life Sciences, Northeast Agricultural University, Harbin 150030, China; (Y.Q.); (Y.Z.) |
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| Abstract: | Understanding the interaction between proteins and polyphenols is of significance to food industries. The aim of this research was to investigate the mode of aggregation for trypsin-EGCG (Epigallocatechin-3-gallate) complexes. For this, the complex was characterized by fluorescence spectroscopy, circular dichroism (CD) spectra, small-angel X-ray scattering (SAXS), and atomic force microscope (AFM) techniques. The results showed that the fluorescence intensity of trypsin-EGCG complexes decreased with increasing the concentration of EGCG, indicating that the interaction between trypsin and EGCG resulted in changes in the microenvironment around fluorescent amino acid residues. The results of CD analysis showed conformational changes in trypsin after binding with EGCG. The results from SAXS analysis showed that the addition of EGCG results in the formation of aggregates of trypsin-EGCG complexes, and increasing the concentration of EGCG resulted in larger aggregates. AFM images showed that the trypsin-EGCG complex formed aggregates of irregular ellipsoidal shapes with the size of about 200 × 400 × 200 nm, with EGCG interconnecting the trypsin particles. Overall, according to these results, it was concluded that the large aggregates of trypsin-EGCG complexes are formed from several small aggregates that are interconnected. The results of this study shed some light on the interaction between digestive enzymes and EGCG. |
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| Keywords: | trypsin EGCG aggregates small-angel X-ray scattering polyphenols |
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