Effect of Hydrated Ionic Liquid on Photocycle and Dynamics of Photoactive Yellow Protein |
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| Authors: | Utana Umezaki Miu Hatakenaka Kana Onodera Hiroto Mizutani Suhyang Kim Yusuke Nakasone Masahide Terazima Yoshifumi Kimura |
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| Institution: | 1.Department of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University, Kyotanabe 610-0321, Japan; (U.U.); (M.H.);2.Department of Applied Chemistry, Graduate School of Science and Engineering, Doshisha University, Kyotanabe 610-0321, Japan; (K.O.); (H.M.);3.Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan; (S.K.); (Y.N.); (M.T.) |
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| Abstract: | The mechanism by which proteins are solvated in hydrated ionic liquids remains an open question. Herein, the photoexcitation dynamics of photoactive yellow protein dissolved in hydrated choline dihydrogen phosphate (Hych]dhp]) were studied by transient absorption and transient grating spectroscopy. The photocyclic reaction of the protein in Hych]dhp] was similar to that observed in the buffer solution, as confirmed by transient absorption spectroscopy. However, the structural change of the protein during the photocycle in Hych]dhp] was found to be different from that observed in the buffer solution. The known change in the diffusion coefficient of the protein was apparently suppressed in high concentrations of ch]dhp], plausibly due to stabilization of the secondary structure. |
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| Keywords: | hydrated ionic liquid photoactive yellow protein photocycle transient grating spectroscopy conformational change |
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