Five Surfactin Isomers Produced during Cheonggukjang Fermentation by Bacillus pumilus HY1 and Their Properties |
| |
Authors: | Su-Young Hong Dong-Hee Lee Jin-Hwan Lee Md Azizul Haque Kye-Man Cho |
| |
Institution: | 1.SBT Business Division, Food Science Research Institute, Kolmar BNH Inc., Sejong 30003, Korea;2.Industry Academy Cooperation Foundation, Andong National University, Andong 36729, Korea;3.Department of Life Resources Industry, Dong-A University, Busan 49315, Korea;4.Department of Biochemistry & Molecular Biology, Hajee Mohammad Danesh Science & Technology University, Dinajpur 5200, Bangladesh;5.Department of Food Science, Gyeongsang National University, Jinju 52725, Korea |
| |
Abstract: | The cyclic lipopeptide produced from Bacillus pumilus strain HY1 was isolated from Korean soybean sauce cheonggukjang. The chemical structures of the surfactin isomers were analyzed using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS). The five potential surfactin isoforms were detected with protonated masses of m/z 994.7, 1008.7, 1022.7, 1036.7, and 1050.7 and different structures in combination with Na+, K+, and Ca2+ ions. ESI-MS/MS analysis revealed that the isolated surfactin possessed the precise amino acid sequence LLVDLL and hydroxyl fatty acids with 12 to 16 carbons. The surfactin content during cheonggukjang fermentation increased from 0.3 to 51.2 mg/kg over 60 h of fermentation. The mixture of five surfactin isoforms of cheonggukjang inhibited the growth of two cancer cell lines. The growth of both MCF-7 and Caco-2 cells was strongly inhibited with 100 μg/μL of surfactin. This study is the first-time report of five surfactin isomers of Bacillus pumilus strain HY1 during Korean soybean sauce cheonggukjang fermentation, which has cytotoxic properties. |
| |
Keywords: | Bacillus pumilus HY1 solid-state fermentation cheonggukjang surfactin mass spectrum cytotoxic effect |
|
|