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Fluorescent turn-on detection of cysteine using a molecularly imprinted polyacrylate linked to allylthiol-capped CdTe quantum dots
Authors:Mu-Rong Chao  Chiung-Wen Hu  Jian-Lian Chen
Institution:2. Department of Occupational Safety and Health, Chung Shan Medical University, Taichung, 402, Taiwan
3. Department of Occupational Medicine, Chung Shan Medical University Hospital, Taichung, 402, Taiwan
4. Department of Public Health, Chung Shan Medical University, Taichung, 402, Taiwan
5. Department of Family and Community Medicine, Chung Shan Medical University Hospital, Taichung, 402, Taiwan
1. School of Pharmacy, China Medical University, No. 91 Hsueh-Shih Road, Taichung, 40402, Taiwan
Abstract:CdTe quantum dots capped with thioglycolic acid (TGA) display a strong turn-on fluorescence response if exposed to solutions of cysteine (Cys). In order to exploit this effect, a molecularly imprinted polymer (MIP) for Cys was covalently linked to the QDs via allyl mercaptan. The resulting nanomaterials (QDs, MIP-coated QDs, and nonimprint-coated QDs) were characterized by FTIR and scanning electron microscopy. The adsorption of Cys was studied in phosphate buffer (pH 7.4) with respect to equilibration times (5, 15, and 40 min, respectively), binding constants 2.98, 2.42, and 0.96 (×104 M?1)], and Langmuir isotherms (R2?=?0.9995, 0.9999, and 0.9983) in the Cys concentration range between 3.33 μM to 500 μM. The method has a detection limit of 0.85 μM (3σ, blank, for n?=?10). The selectivity of the MIP-coated QDs for Cys over 19 other amino acids is similar to that of bare QDs, but MIP-QDs afford better recoveries of Cys from solutions also containing bovine serum albumin (90 %) and fetal bovine serum (97 %), respectively, when compared to the recoveries that are obtained with bare (non-imprinted) QDs (135 % and 120 %). This is probably due to the fact that the outer MIP shell largely reduces protein wrapping, dot aggregation, and matrix inclusion.
Figure
Using the turn-on fluorescence detection of cysteine template, quantum dots (QDs) conjugated with MIP via allyl mercaptan were synthesized in a one-pot polymerization and exhibited a higher binding selectivity in the presence of serum matrices compared to bare QDs and non-imprinted polymer-QDs.
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