INVESTIGATION OF THE ANTIVIRAL ACTION OF THE PHOTOACTIVE COMPOUND PHENYLHEPTATRIYNE |
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| Authors: | J. B. Hudson E. A. Graham G. H. N. Towers |
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| Affiliation: | Division of Medical Microbiology, University of British Columbia Vancouver, B.C. Canada, V6T 1W5;Department of Botany, University of British Columbia Vancouver, B.C. Canada, V6T 1W5 |
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| Abstract: | Abstract— Murine cytomegalovirus, a herpes virus, was used as a model virus to investigate the mechanism of the anti-viral action of phenylheptatriyne in long wave ultraviolet light. The genome and proteins of the inactivated virus penetrated the nuclei of susceptible cells normally. Furthermore, the viral genome did not contain extra single strand breaks or cross-links. However, cells infected with the treated virus did not synthesize late viral proteins, as determined by polyacrylamide gel electrophoresis. nor did they synthesize late viral RNA and viral DNA according to nucleic acid hybridization tests. Thus the compound may interfere with an early viral function so that the replication cycle cannot proceed. |
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