Decolorization of Some Azo Dyes by Immobilized <Emphasis Type="Italic">Geotrichum</Emphasis> sp. Biomass in Fluidized Bed Bioreactor |
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Authors: | Youssef Zeroual Beom Su Kim Myoung Won Yang Mohamed Blaghen Kang Min Lee |
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Institution: | (1) Laboratory of Enzyme Technology, College of Natural Science, Chonbuk National University, Chonju, 561-756, Republic of Korea;(2) Laboratory of Microbiology, Biotechnology and Environment, Faculty of Sciences, University Hassan II, Cassablanca, Morocco |
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Abstract: | Geotrichum sp. strain, which is able to decolorize azo dyes enzymatically, was used in this study for decolorization of synthetics solutions
contaminated by toxic azo dyes orange G, trypan blue, azorubine, and methyl red. The biomass of Geotrichum sp. was immobilized in calcium alginate and polyacrylamide gels and used for the decolorization of tested azo dyes in fluidized
bed bioreactor. The highest specific decolorization rate was obtained when the fungal biomass was entrapped in calcium alginate
beads. Immobilized biomass in calcium alginate continuously decolorized azo dyes after eight repeated batch decolorization
experiments without significant loss of activity whereas polyacrylamide immobilized biomass retained only 10% of its activity
after 4 days of incubation. The effects of some physicochemical parameters such as temperature, pH, and dyes concentration
on decolorization performance of isolated fungal strain were also investigated. |
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Keywords: | Decolorization Azo dyes Geotrichum sp Immobilization |
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