Electron Transfer Binding Site of Cytochrome c to Carboxylic Acid-Terminated Alkanethiol Self-assembled Monolayers Immobilized on Gold Electrode |
| |
Authors: | Niki K Pressler K R Sprinkle J R Li H Margoliash E |
| |
Institution: | (1) Department of Chemistry, Illinois State University, Normal, Illinois 61790-4160, USA;(2) Department of Biological and Exercise Sciences, Northeastern Illinois University, Chicago, Illinois, USA;(3) Department of Biological Sciences, The University of Illinois at Chicago, Chicago, Illinois 60607-7020, USA |
| |
Abstract: | It is proposed that Lys-13 of mammalian cytochrome cfacilitates the most efficient electron transfer (ET) pathway to the carboxylate terminus of alkanethiol self-assembled monolayers (SAM) on gold electrodes. In order to confirm the proposed ET pathway, the ET reaction rate of a rat cytochrome c mutant (RC9K13A), in which lysine-13 is replaced by alanine, is measured at a 3-mercaptopropionate SAM on a gold electrode. The ET rate of K13A is more than six orders of magnitude smaller than that of the native one. In the mutant, Ala-13 can no longer facilitate the ET pathway. Based on the measurements, the potential candidate for the binding site of RC9K13A is Lys-8. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|