Natural Sphingomonas glycolipids vary greatly in their ability to activate natural killer T cells |
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Authors: | Kinjo Yuki Pei Bo Bufali Simone Raju Ravinder Richardson Stewart K Imamura Masakazu Fujio Masakazu Wu Douglass Khurana Archana Kawahara Kazuyoshi Wong Chi-Huey Howell Amy R Seeberger Peter H Kronenberg Mitchell |
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Affiliation: | Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA. |
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Abstract: | Mouse natural killer T (NKT) cells expressing an invariant T cell antigen receptor (TCR) recognize glycosphingolipids (GSLs) from Sphingomonas bacteria. The synthetic antigens previously tested, however, were designed to closely resemble the potent synthetic agonist alpha-galactosyl ceramide (alphaGalCer), which contains a monosaccharide and a C18:0 sphingosine lipid. Some Sphingomonas bacteria, however, also have oligosaccharide-containing GSLs, and they normally synthesize several GSLs with different sphingosine chains including one with a cyclopropyl ring-containing C21:0 (C21cycl) sphingosine. Here we studied the stimulation of NKT cells with synthetic GSL antigens containing natural tetrasaccharide sugars, or the C21cycl sphingosine. Our results indicate that there is a great degree of variability in the antigenic potency of different natural Sphingomonas glycolipids, with the C21cycl sphingosine having intermediate potency and the oligosaccharide-containing antigens exhibiting limited or no stimulatory capacity. |
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Keywords: | CHEMBIO MOLIMMUNO |
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