Effect of phalloidin on the skeletal muscle ADP-actin filaments |
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Authors: | Réka Dudás Tünde Kupi Andrea Vig J. Orbán D. Lőrinczy |
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Affiliation: | (1) Department of Biophysics, University of Pécs, Medical School, Szigeti str. 12, Pécs, 7624, Hungary |
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Abstract: | The effect of phalloidin on the thermal stability of skeletal actin filaments polymerized from ADP-binding monomers was investigated with the method of differential scanning calorimetry. Phalloidin shifted the melting temperature of the ADP-F-actin from 59.1±1.0 to 80.0±1.2°C. The stabilizing effect of phalloidin propagated cooperatively along the filament. The cooperativity factor according to the applied model was 1.07±0.11. With these measurements it was possible to demonstrate that the binding of phalloidin has lower influence on the adjacent protomers in ADP- (k=1) than in ATP-actin filaments (k=3). |
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Keywords: | ADP-actin filament calorimetry phalloidin stability thermodynamics |
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