| GENETIC ENGINEERING OF TOBACCO PLANTS WITH CMV RESISTANCE CONFERRED BY MONOMER cDNA OF SATELLITE RNA |
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| 引用本文： | 吴世宣,赵淑珍,王革娇,杨希才,张春霞,王昕,田波. GENETIC ENGINEERING OF TOBACCO PLANTS WITH CMV RESISTANCE CONFERRED BY MONOMER cDNA OF SATELLITE RNA[J]. 中国科学B辑(英文版), 1992, 0(6) |
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| 作者姓名： | 吴世宣 赵淑珍 王革娇 杨希才 张春霞 王昕 田波 |
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| 作者单位： | Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC，Institute of Microbiology，Academia Sinica，Beijing 100080，PRC |
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| 摘 要： | The synthesized full-length cDNA to CMV satellite RNA-1 was integrated into plant expression vector RoKII with a CaMV 35S promoter. Infected by Agrobacterium tumefaciens harbouring the recombinant plasmid. The tobacco leaf discs (the G-140 variety which is widely cultivated in China) were regenerated into plants. After being inoculated with virulent strain CMV, most of the transgenic plants expressed satellite RNA at high levels and developed a much milder symptom than the untransformed ones.Basically in accordance with Mendel's law of segregation, the novel tobacco pure line engineered with viral resistance was screened out. The secondary generation transgenic plants still maintained high level satellite RNA expression and the resistance to CMV.
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GENETIC ENGINEERING OF TOBACCO PLANTS WITH CMV RESISTANCE CONFERRED BY MONOMER cDNA OF SATELLITE RNA |
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| WU SHI-XUAN ZHAO SHU-ZHEN WANG GE-JIAO YANG XI-CAI ZHANG CHUN-XIA WANG XIN AND TIAN BO. GENETIC ENGINEERING OF TOBACCO PLANTS WITH CMV RESISTANCE CONFERRED BY MONOMER cDNA OF SATELLITE RNA[J]. Science in China(Chemistry), 1992, 0(6) |
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| Authors: | WU SHI-XUAN ZHAO SHU-ZHEN WANG GE-JIAO YANG XI-CAI ZHANG CHUN-XIA WANG XIN AND TIAN BO |
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| Abstract: | The synthesized full-length cDNA to CMV satellite RNA-1 was integrated into plant expression vector RoKII with a CaMV 35S promoter. Infected by Agrobacterium tumefaciens harbouring the recombinant plasmid. The tobacco leaf discs (the G-140 variety which is widely cultivated in China) were regenerated into plants. After being inoculated with virulent strain CMV, most of the transgenic plants expressed satellite RNA at high levels and developed a much milder symptom than the untransformed ones.Basically in accordance with Mendel's law of segregation, the novel tobacco pure line engineered with viral resistance was screened out. The secondary generation transgenic plants still maintained high level satellite RNA expression and the resistance to CMV. |
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| Keywords: | CMV (cucumber mosaicvirus) satellite RNA transgenic plant viral resistance. |
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