A new anion-sensitive biosensor using an ion-sensitive field effect transistor and a light-driven chloride pump,halorhodopsin |
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Authors: | Atsushi Seki Izumi Kubo Hiroyuki Sasabe Hiroaki Tomioka |
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Institution: | (1) Department of Bioengineering, Faculty of Engineering, Soka University, 1-236 Tangi-cho, Hachioji, 192 Tokyo, Japan;(2) Laboratory for Nano-photonics, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Hirosawa, Wako-shi, 351-01 Saitama, Japan |
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Abstract: | A new biosensor sensitive to chloride anion using a light-driven chloride pump protein, halorhodopsin (hR), and an ion-sensitive
field effect transistor (ISFET) has been developed. Membrane vesicles of halophilic bacteria containing hR were immobilized
in the matrix of polyvinylbutyral resin on the surface of the ISFET. The gate voltage of this device changed in the min time
scale under yellow light illumination. The response for chloride anion increased according to the increase of chloride anion
concentration in the bulk aqueous phase. In the dark, the gate potential did not change even in the presence of chloride anion.
These chloride-dependent gate potential changes of the hR-ISFET indicate that the chloride pumping by hR is active on the
ISFET and that ISFET detects the light-dependent chloride transport by hR. |
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Keywords: | Ion-sensitive field effect transistor (ISFET) halorhodopsin halophilic bacteria chloride anion ion-pump protein |
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