Study of electrochemical oxidation of cyanidin glycosides by online combination of electrochemistry with electrospray ionization tandem mass spectrometry |
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Authors: | David Jirovsky Petr Bedná? Renáta Myjavcová Zdenka Barto?ová Jana Skopalová Michaela Tvrdoňová and Karel Lemr |
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Institution: | (1) Department of Analytical Chemistry, Palack? University, 17. Listopadu 1192/12, 771 46 Olomouc, Czech Republic |
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Abstract: | Abstract Electrochemistry coupled with mass spectrometry (EC–MS) is a promising analytical tool for the online study of oxidation processes
of anthocyanins. Two cyanidin glycosides, cyanidin-3-galactoside (ideain) and cyanidin-3,5-diglucoside (cyanin), were subjected
to electrochemical oxidation and subsequent online mass spectrometric identification of the formed products. Application of
relatively low working potentials (around 100 mV vs. Pd/H2) using a porous graphite coulometric electrode yielded detectable oxidation products. As determined by hydrodynamic voltammetry,
the monoglycosylated analogue undergoes anodic oxidation easier than the diglycosylated one. As a first step of the electrochemically
induced oxidation, incorporation of a hydroxyl group was observed for both glycosides. Besides, an oxidative condensation
of two anthocyanin molecules was observed. The proposed oxidative condensation was further confirmed by consecutive fragmentation
(i.e., collection of MS2, MS3, and MS4 spectra) in which corresponding subsequent losses of the sugar moiety were observed. |
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