Surface conductivity of biological macromolecules measured by nanopipette dielectrophoresis |
| |
Authors: | Clarke Richard W Piper Joe D Ying Liming Klenerman David |
| |
Affiliation: | Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, United Kingdom. |
| |
Abstract: | We report the measurement of the surface conductivity of biological macromolecules by dielectrophoretic trapping at the tip of a glass nanopipet. We find that the threshold voltage for trapping is a function of salt concentration and can be directly linked to the effective conductivity of the biomolecule and its solvation shell. The surface conductivities obtained for 20-mer single-stranded DNA, 40-mer double-stranded DNA, and yellow fluorescent protein are 7.9+/-1.9 nS, 5.3+/-0.7 nS, and 21.5+/-1.6 nS, respectively. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|