Large-scale separation of resveratrol, anthraglycoside A and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc by high-speed counter-current chromatography. |
| |
| Authors: | F Yang T Zhang Y Ito |
| |
| Affiliation: | Beijing Research Centre for Separation and Purification of Natural Products, Beijing Institute of New Technology Application, China. |
| |
| Abstract: | High-speed counter-current chromatography was successfully applied to the large-scale separation of resveratrol, anthraglycoside A and anthraglycoside B from the crude extract of Polygonum cuspidatum Sieb. et Zucc using a two-phase solvent system composed of chloroform, methanol and water. Resveratrol, anthraglycoside A and anthraglycoside B were separated from multigram quantities (5 g) of crude extract of P. cuspidatum. The separation yielded 200 mg to 1 g of these three compounds each at over 98% purity as determined by HPLC. The chemical structures of these components were identified by nuclear magnetic resonance (NMR) and MS. |
| |
| Keywords: | |
|
|
