Pedal to the Metal: The Homogeneous Catalysis of the Native Chemical Ligation Reaction |
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Authors: | Dr Vincent Diemer Olga Firstova Dr Vangelis Agouridas Dr Oleg Melnyk |
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Institution: | Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 –, UMR 9017 –, CIIL –, Center for Infection and Immunity of Lille, 59000 Lille, France |
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Abstract: | The native chemical ligation reaction of peptide thioesters with cysteinyl peptides is a pivotal chemical process in the production of native or modified peptides and proteins, and well beyond in the preparation of various biomolecule analogs and materials. To benefit from this reaction at its fullest and to access all the possible applications, the experimentalist needs to know the factors affecting its rate and how to control it. This concept article presents the fundamental principles underlying the rate of the native chemical ligation and its homogeneous catalysis by nucleophiles. It has been prepared to serve as a quick guide in the search for an appropriate catalyst. |
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Keywords: | chemoselectivity desulfurization homogeneous catalysis native chemical ligation nucleophilic catalysis thiol-thioester exchange |
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