Fast Cysteine Bioconjugation Chemistry |
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Authors: | Dr. Fa-Jie Chen Dr. Jianmin Gao |
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Affiliation: | Department of Chemistry, Boston College, Merkert Chemistry Center, 2609 Beacon Street, Chestnut Hill, MA 02467 USA |
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Abstract: | Cysteine bioconjugation serves as a powerful tool in biological research and has been widely used for chemical modification of proteins, constructing antibody-drug conjugates, and enabling cell imaging studies. Cysteine conjugation reactions with fast kinetics and exquisite selectivity have been under heavy pursuit as they would allow clean protein modification with just stoichiometric amounts of reagents, which minimizes side reactions, simplifies purification and broadens functional group tolerance. In this concept, we summarize the recent advances in fast cysteine bioconjugation, and discuss the mechanism and chemical principles that underlie the high efficiencies of the newly developed cysteine reactive reagents. |
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Keywords: | cysteine bioconjugation fast kinetics N-terminal cysteine protein modification reaction rate constant |
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