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Tailoring the NIR-II Photoluminescence of Single Thiolated Au25 Nanoclusters by Selective Binding to Proteins** |
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| Authors: | Dr. Franck Bertorelle Dr. K. David Wegner Martina Perić Bakulić Hussein Fakhouri Clothilde Comby-Zerbino Dr. Amin Sagar Dr. Pau Bernadó Dr. Ute Resch-Genger Prof. Vlasta Bonačić-Koutecký Dr. Xavier Le Guével Dr. Rodolphe Antoine |
| Affiliation: | 1. Institut Lumière Matière, UMR5306, Université Claude Bernard Lyon1–CNRS, Université de Lyon, 69622 Villeurbanne Cedex, France Nantes Université, CNRS, US2B, UMR 6286, 44000 Nantes, France;2. Federal Institute for Materials Research and Testing (BAM), Richard-Willstaetter-Str. 11, 12489 Berlin, Germany;3. Center of Excellence for Science and Technology, Integration of Mediterranean Region (STIM) at, Interdisciplinary Center for Advanced Sciences and Technology (ICAST), University of Split, Poljička cesta 35, 21000 Split, Croatia;4. Institut Lumière Matière, UMR5306, Université Claude Bernard Lyon1–CNRS, Université de Lyon, 69622 Villeurbanne Cedex, France Center of Excellence for Science and Technology, Integration of Mediterranean Region (STIM) at, Interdisciplinary Center for Advanced Sciences and Technology (ICAST), University of Split, Poljička cesta 35, 21000 Split, Croatia;5. Institut Lumière Matière, UMR5306, Université Claude Bernard Lyon1–CNRS, Université de Lyon, 69622 Villeurbanne Cedex, France;6. Centre de Biologie Structurale, Université de Montpellier, INSERM, CNRS, 29 rue de Navacelles, 34090 Montpellier, France;7. Institute for Advanced Biosciences, Université Grenoble Alpes/ INSERM1209/CNRS-UMR5309, 38700 La Tronche, France |
| Abstract: | Atomically precise gold nanoclusters are a fascinating class of nanomaterials that exhibit molecule-like properties and have outstanding photoluminescence (PL). Their ultrasmall size, molecular chemistry, and biocompatibility make them extremely appealing for selective biomolecule labeling in investigations of biological mechanisms at the cellular and anatomical levels. In this work, we report a simple route to incorporate a preformed Au25 nanocluster into a model bovine serum albumin (BSA) protein. A new approach combining small-angle X-ray scattering and molecular modeling provides a clear localization of a single Au25 within the protein to a cysteine residue on the gold nanocluster surface. Attaching Au25 to BSA strikingly modifies the PL properties with enhancement and a redshift in the second near-infrared (NIR-II) window. This study paves the way to conrol the design of selective sensitive probes in biomolecules through a ligand-based strategy to enable the optical detection of biomolecules in a cellular environment by live imaging. |
| Keywords: | gold luminescence nanoclusters proteins SWIR/NIR-II |