高分子覆盖型硅胶填料的制备与色谱保留行为

 采用甲基丙烯酸甲酯 (MMA)或MMA 亚乙基二甲基丙烯酸酯 (EDMA)在硅胶表面聚合的方法 ,制备生成了新型高分子覆盖型硅胶填料C或D。借助红外光谱、元素分析、尺寸排阻色谱和反相液相色谱分析对反应过程、覆盖程度、交联剂的影响和填料的色谱保留行为进行了评价和讨论。结果显示 ,在合成时可以通过控制聚合单体的量控制生成高分子层的厚度 ,而合成中加入交联剂可以改变填料表面的微孔构造。通过考察这种色谱填料的疏水性和对芳香族化合物的分离性能 ,认为其柱效和分离效果接近C18填料的性能。

Syntheses of Polymer Coated Silica Packing Materials and Their Retention Behaviors for Reversed-Phase Liquid Chromatography

The polymer coated silica packings C or D with methylmethacrylate(MMA) or MMA ethylene dimethacrylate(EDMA) monomer were prepared For the packing materials the polymer coated degree, effects of cross linking agent and retention behavior were characterized by diffuse reflectance infrared Fourier transform spectroscopy, elemental analysis, size exclusion chromatography and reversed phase liquid chromatography The results showed that polymer coated degree on silica depended on the amount of monomer, and the volume of micropore of the meterials, which was controlled more by the cross linked structure than by the linear structure The hydrophobicity, retention ratio and number of theoretical plate (80% methanol and 60% acetonitrile) of the packing materials in comparison with those of C 18 are discussed The polymer coated silica packings C and D showed the same stereoselectivity for separation of aromatic compounds, such as triptycene.