| 反相离子对色谱-脉冲安培电化学法测定硫酸庆大霉素中各组分含量 |
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| 引用本文： | 习玲玲,朱岩. 反相离子对色谱-脉冲安培电化学法测定硫酸庆大霉素中各组分含量[J]. 分析化学, 2006, 34(12): 1763-1766 |
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| 作者姓名： | 习玲玲 朱岩 |
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| 作者单位： | 浙江大学西溪校区化学系,杭州,310028 |
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| 基金项目： | 国家自然科学基金项目(No.20375025)，浙江省自然科学基金(No.Z404105)，教育部和浙江省留学回国人员科研启动基金，教育部和浙江省留学回国人员分析测试基金资助课题 |
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| 摘 要： | 建立了一种新的用反相离子对液相色谱(LC)分离,以金电极为工作电极的脉冲安培电化学法(PAD)直接检测硫酸庆大霉素中各组分含量的分析方法。流动相为0.033 mol/L草酸、0.012 mol/L七氟丁酸、210 mL/L乙睛,用稀NaOH调节pH至3.4。与报道的其它方法相比,该方法能使庆大霉素各有效组分C1、C1 a、C2、C2 a很好分离,整个分析过程<30 m in。考察了各色谱参数对分离测定的影响。实验证明,本方法不需要衍生化,可直接检测硫酸庆大霉素。
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| 关 键 词： | 庆大霉素 脉冲安培电化学检测 液相色谱 |
| 收稿时间： | 2006-03-21 |
| 修稿时间： | 2006-03-212006-07-10 |
Analysis of Gentamicin Sulfate by Liquid Chromatography with Pulsed Amperometric Detection |
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| Xi Lingling,Zhu Yan. Analysis of Gentamicin Sulfate by Liquid Chromatography with Pulsed Amperometric Detection[J]. Chinese Journal of Analytical Chemistry, 2006, 34(12): 1763-1766 |
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| Authors: | Xi Lingling Zhu Yan |
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| Affiliation: | Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028 |
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| Abstract: | A reversed phase ion pair liquid chromatography(LC) method with pulsed amperometric detection on a gold electrode after post added NaOH is described.The mobile phase consisted of an aqueous solution containing 0.033 mol/L oxalic acid,0.012 mol/L heptafluorobutyric acid and 210 mol/L acetonitrile with pH 3.40 adjusting by dilute NaOH solution.The total analysis time was less than 30 min,and the method allows a better separation of gentamicin C_(1),C_(1a),C_(2),and C_(2a).The effects of the different chromatographic(parameters) on the separation were also investigated. |
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| Keywords: | Gentamicin pulsed amperometric detection liquid chromatography |
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