| 萝卜红花色苷的高效液相色谱法测定 |
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| 引用本文： | 游辉,欧阳杰,武彦文,杨寅,汪雨,陈舜琮.萝卜红花色苷的高效液相色谱法测定[J].分析测试学报,2010,29(3). |
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| 作者姓名： | 游辉 欧阳杰 武彦文 杨寅 汪雨 陈舜琮 |
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| 作者单位： | 1. 国产科学仪器应用示范中心,北京,100089
2. 北京林业大学,生物科学与技术学院食品科学与工程系,北京,100083 |
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| 基金项目： | 国家自然科学基金资助项目，科技部创新方法工作专项资助项目 |
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| 摘 要： | 建立了高效液相色谱法测定萝卜红色素中天竺葵-3-O-葡萄糖苷含量的方法。样品用甲醇溶解,净化后用高效液相色谱分析。结果显示,选择合适的流动相可以获得较好的分离效果。实验采用Ultimate XB-C18色谱柱(4.6 mm×250 mm×5μm),乙腈-5%甲酸溶液(体积比24∶76)为流动相,流速1.0 mL/min,柱温为25℃,紫外检测波长为525 nm,进样量为10μL。实验表明,天竺葵-3-O-葡萄糖苷在0.01~0.10 g/L范围内线性关系良好,r=0.999,平均加标回收率为97%~100%,检出限为0.002 5 g/L。方法灵敏、准确、样品处理简单,适用于萝卜红色素中花色苷含量的测定。
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| 关 键 词： | 萝卜红 天竺葵-3-O-葡萄糖苷 高效液相色谱 |
High Performance Liquid Chromatography Determination of Pelargonidin-3-O-glucoside in Radish Red |
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| YOU Hui,OUYANG Jie,WU Yan-wen,YANG Yin,WANG Yu,CHEN Shun-cong.High Performance Liquid Chromatography Determination of Pelargonidin-3-O-glucoside in Radish Red[J].Journal of Instrumental Analysis,2010,29(3). |
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| Authors: | YOU Hui OUYANG Jie WU Yan-wen YANG Yin WANG Yu CHEN Shun-cong |
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| Institution: | YOU Hui1,OUYANG Jie2,WU Yan-wen1,YANG Yin1,WANG Yu1,CHEN Shun-cong1(1.Application & Demonstration Center for Homegrown Scientific Instruments,Beijing 100089,China,2.Department of Food Science , Engineering,College of Biological Sciences , Technology,Beijing Forestry University,Beijing 100083,China) |
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| Abstract: | A high performance liquid chromatographic(HPLC) method was developed for determination of pelargonidin-3-O-glucoside in Radish Red samples.The sample was dissolved in methanol,and separated by a Ultimate XB-C_(18) (4.6 mm×250 mm×5 μm) column using acetonitrile-5% formic acid (24 : 76,by volume) as a mobile phase with flow rate of 1.0 mL/min at column temperature of 25 ℃.The UV detection wavelength was set at 525 nm.A good linearity was obtained in the range of 0.01-0.10 g/L with correlation coefficient of 0.999.The average spiked recoveries of pelargonidin-3-O-glucoside was 97%-100%.The limit of detection for the method was 0.002 5 g/L.The results indicated that the method was simple,sensitive and accurate,and could be applied in the determination of pelargonidin-3-O-glucoside in Radish Red. |
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| Keywords: | Radish Red pelargonidin-3-O-glucoside high performance liquid chromatography(HPLC) |
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