Acid Pullulanase from Bacillus polymyxa MIR-23 |
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| Authors: | Guillermo R. Castro Luis M. Ducrey Santopietro Faustino Siñeriz |
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| Affiliation: | (1) Cátedra de Microbiologia Superior, Facultad de Bioquimica, Quimica y Farmacia, Universidad Nacional de Tucuman, PROIMI-MIRCEN. Avda. Belgrano y Caseros, 4000 Tucumán;(2) Laboratorio de Microbiologia, Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina |
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| Abstract: | Within the frame of a screening program aimed at the isolation of amylolytic sporeformers, one strain with high amylolytic activity designated MIR-23 was selected. The microbial characterization was carried out by morphological and biochemical tests and, by means of statistical treatment, was identified asBacillus polymyxa. The organism could grow in acidic conditions (pH 5.0) on a starch medium and produce α-amylase, pullulanase, and α-glucosidase. Batch cultures showed the highest enzyme activities in the stationary phase. Pullulanase activity exhibited an optimal temperature of 52–57°C at pH 4.5–5.5. These properties would allow its use in the saccharification processes in the starch industries. |
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| Keywords: | Bacillus polymyxa pullulanase, α -amylase α -glucosidase starch degradation batch culture acidophilic microorganism |
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