The application of retention indices using the alkylarylketone scale to the separation of the barbiturates by HPLC. II The effect of the stationary phase

Summary The capacity factors and retention indices, based on the alkylarylketone scale, of ten barbiturates and a set of five column test compounds have been compared on a series of different octadecylsilyl bonded (ODS) silica columns under identical elution conditions of 40:60 methanol-buffer pH 8.5 at 30 °C. The retentions were highly reproducible on columns prepared from the same batch or different batches of ODS-Hypersil, but large differences in the capacity factors were found when column packings from different manufacturers were compared. Retention indices were more reproducible than capacity factors but they could not compensate for differences between the different packing materials. Presented at the 15th International Symposium on Chromatography, Nürnberg, October 1984