框架核酸高通量检测芯片

构建了一种基于框架核酸的高通量生物检测芯片.利用超微量移液自动化平台,将包含框架核酸探针的液滴按照预设命令固定至生物芯片微阵列上,在探针捕获核酸靶标后利用集成的基因芯片扫描仪对芯片进行成像,通过分析荧光强度定量化分析靶标浓度.结果表明,此框架核酸芯片能够实现框架核酸探针的高通量制备, 24 h即可制备具有15万个点的微阵列,且点间距离的相对偏差W≤10%、荧光强度的变异系数CV=3.30%,具有较高的稳定性,远高于国家标准.此外,该芯片具备高灵敏度、可寻址的高通量生物分析能力,对核酸靶标的检测限可达100 pmol/L.随着多种探针技术的发展,生物检测微阵列技术在高通量生物分析领域展示出巨大的潜力.

High-throughput Biological Microarrays Based on Framework Nucleic Acids

A high-throughput biological microarray based on framework nucleic acid（FNA） was constructed. Using an automated ultra-micro pipetting platform， the droplets containing FNAs-based probes were fabricated onto the glass substrates according to the pre-defined commands. After the targets were captured by the probes， the micro- arrays were scanned by the integrated gene chip scanner， the results of which could be further used for the quantifi- cation of target concentration. It was found that the FNA probes could be prepared with high throughput on the bio- logical microarrays， and the microarrays with 150000 spots can be prepared within 24 h. Moreover， the relative deviation（W） of distance between the points is less than 10%， and the coefficient of variation（CV） of fluorescence intensity is 3.30%. The properties of each spot on the microarrays were stable， which is much higher than the national standard. Additionally， we demonstrated that the FNA-based microarray possessed high addressability， high sen- sitivity and high-throughput capabilities. The limit of detection for nucleic acid target was down to 100 pmol/L. With the progress of various probe technologies， the microarray platform shows great potential for bioanalysis.