Amperometric glucose biosensor based on a gold nanorods/cellulose acetate composite film as immobilization matrix |
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Authors: | Xiangling Ren Dong Chen Xianwei Meng Fangqiong Tang Aiming Du Lin Zhang |
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Affiliation: | aKey Laboratory of Photochemical Conversion and Optoelectronic Materials, Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No. 2 Beiyitiao Zhongguancun, Beijing 100190, People's Republic of China |
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Abstract: | We report on the utilization of gold nanorods to create a highly responsive glucose biosensor. The feasibility of an amperometric glucose biosensor based on immobilization of glucose oxidase (GOx) in gold nanorod is investigated. GOx is simply mixed with gold nanorods and cross-linked with a cellulose acetate (CA) medium by glutaraldehyde. The adsorption of GOx on the gold nanorods is confirmed by X-ray photoelectron spectroscopy (XPS) measurements. Circular dichroism (CD) and UV-spectrum results show that the activity of GOx was preserved after conjugating with gold nanorods. The current response of modified electrode is 10 times higher than that of without gold nanorods. Under optimal conditions, the biosensor shows high sensitivity (8.4 μA cm−2 mM−1), low detection limit (2 × 10−5 M), good storage stability and high affinity to glucose (). A linear calibration plot is obtained in the wide concentration range from 3 × 10−5 to 2.2 × 10−3 M. |
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Keywords: | Gold nanorods Immobilization enzyme Glucose oxidase Biosensor |
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