对苏丹红GB/T19681-2005检测方法的探讨与改进

探讨苏丹红国标检测方法存在的问题并在该方法的基础上进行改进。用正己烷提取样品中的苏丹红染料,通过中性氧化铝小柱富集并去除杂质和内源性色素的干扰,高度外标法定量。在选定的分离条件下,苏丹红Ⅰ~Ⅳ色谱峰分离良好,检出限分别为8、9、9、10μg/kg,测定结果相对标准偏差分别为0.3%、0.2%、0.1%、0.1%(n=6)。苏丹红Ⅰ~Ⅳ的浓度分别在2.16~7.56、2.02~7.07、2.0~7.0、2.08~7.28μg/mL范围内与响应值具有良好的线性关系,线性相关系数分别为0.999 9、0.999 8、0.999 9、0.999 9。改进后的方法适合基层检验机构使用。

DISCUSSION AND IMPROVEMENT OF GB/T 19681-2005 EXAMINATION METHOD FOR SUDAN RED

GB/T 19681 -2005 examination method for sudan red was discussed and improved. Sudan red dye was extracted by hexane extraction, the impurity and the endocardial pigment disturbance in which were concentrated and removed by neutral aluminum oxide column and quantified by highly external standard method. Under the designation separation conditions, the sudan red Ⅰ～Ⅳ chromatograph peak separation was fine, the detection limits were 8, 9, 9, 10 ug/kg, respectively. The linearity range for sudan red Ⅰ～Ⅳ were 2. 16 -7.56, 2.02 -7.07, 2.0 -7.0, 2.08 -7.28 ug/mL with linear correlation coefficient of 0.9999, 0.9998, 0.9999, 0.9999, respectively. The improvement method is suitable for the basic unit examination organization.