| 化学发光酶联免疫分析检测血清中麻疹病毒抗体 |
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| 引用本文： | 章竹君 程明洁. 化学发光酶联免疫分析检测血清中麻疹病毒抗体[J]. 高等学校化学学报, 1994, 15(4): 515-517 |
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| 作者姓名： | 章竹君 程明洁 |
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| 作者单位： | 陕西师范大学分析科学研究所，陕西省卫生防疫站 |
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| 摘 要： | 化学发光酶联免疫分析检测血清中麻疹病毒抗体章竹君，邹克渭，程明洁（陕西师范大学分析科学研究所，西安，７１００６２）（陕西省卫生防疫站）关键词麻疹病毒抗体，化学发光，酶联免疫分析，辣根过氧化物酶目前在计划免疫工作中通常采用间接酶联免疫吸附分析法（ＥＬＩ...
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| 关 键 词： | 麻疹病毒 抗体 ELISA 血清诊断 |
The Determination of Anti-MV Antibody in Serum with CL-ELISA |
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| ZHANG Zhu-Jun, ZOU Ke-Wei. The Determination of Anti-MV Antibody in Serum with CL-ELISA[J]. Chemical Research In Chinese Universities, 1994, 15(4): 515-517 |
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| Authors: | ZHANG Zhu-Jun ZOU Ke-Wei |
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| Abstract: | A method for determination of the anti-MV antibody in serum is established based on chemiluminescence enzyme-linked immunosorbant assay (CL-ELISA). The MV antigen is coated on the polystyrene cup, then reacts with anti-MV serum to form the immunocompound. The anti-HuIgG labeled by HRP is used as a second antibody. The enzymatic activity on the antibody is determined by p-iodophenol enhanced luminol-H2O2 chemiluminescent system, which has higher sensitivity than current chromogenic reaction. The curve of anti-MV antibody title determined with CL-ELISA and ELISA is established and 30 clinical samples are tested. The results show that the CL-ELISA has higher positive ratio than ELISA. Two methods have good correlation. The correlation coefficient is 0.992. |
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| Keywords: | Anti-MV antibody Chemiluminescence Enzyme Immunoassay HRP |
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