| Comparative Analysis of the Volatile Components in the Fresh Roots and Rhizomes of Curcuma wenyujin by Static Headspace Gas Chromatography Mass Spectrometry |
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| 作者姓名： | 方芳 程志红 郭寅龙 蔡延奔 |
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| 作者单位： | Shanghai Mass Spectrometry Center Shanghai Institute of Organic Chemistry，Chinese Academy of Sciences，Shanghai 200032，China，Shanghai Mass Spectrometry Center，Shanghai Institute of Organic Chemistry，Chinese Academy of Sciences，Shanghai 200032，China，Shanghai Mass Spectrometry Center，Shanghai Institute of Organic Chemistry，Chinese Academy of Sciences，Shanghai 200032，China，Wenzhou Institute of Industry and Science Research，Wenzhou，Zhejiang 325028，China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China(No.20475059)，the Chinese Academy of Sciences(No.KGCX2-SW-213-04). |
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| 摘 要： | Static headspace GC-MS method coupled with H/D exchange was firstly developed to determine and identifythe volatile components in the fresh root and rhizome of Curcuma wenyujin.The TIC chromatograms of 3 batchesof fresh roots harvested at different time showed significant difference in the volatile components:the constitutionwas the same but the content of them was different.More than 60 volatile components in fresh roots(Root of C.wenyujin)and rhizomes(Rhizome of C.wenyujin)of C.wenyujin were detected,of which 51 and 48 volatile com-ponents were identified respectively.The fresh roots and rhizomes of C.wenyujin were found to have the similarvolatile components.The contents of these components were calibrated by the response of β-elemene.In addition,the principal active component,β-elemene,was further confirmed and relatively quantified by its standard.γ-terpinene showed obvious allylic hydrogen/deuterium exchange using deuterium oxide which gave a new methodto identify some compounds containing allylic hydrogen.At the same time,the active hydrogen compounds werealso further confirmed.The results show that HS-GC-MS method is a fast,simple and efficient way for the analysisof volatile components from medical plants.
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| 关 键 词： | 姜黄属植物 中药 成分分析 静态顶部空间 GC-MS H/D交换 挥发性成分 气相色谱质谱分析 |
| 收稿时间： | 2006-03-21 |
| 修稿时间： | 2006-03-212006-06-23 |
Comparative Analysis of the Volatile Components in the Fresh Roots and Rhizomes of Curcuma wenyujin by Static Headspace Gas Chromatography Mass Spectrometry |
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| FANG Fang, CHENG Zhi-Hong, GUO Yin-Long, CAI Yan-Ben.Comparative Analysis of the Volatile Components in the Fresh Roots and Rhizomes of Curcuma wenyujin by Static Headspace Gas Chromatography Mass Spectrometry[J].Chinese Journal of Chemistry,2006,24(10):1346-1351. |
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| Authors: | FANG Fang CHENG Zhi-Hong GUO Yin-Long CAI Yan-Ben |
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| Institution: | 1. Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China; 2. Wenzhou Institute oflndustry and Science Research, Wenzhou, Zhejiang 325028, China |
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| Abstract: | Static headspace GC‐MS method coupled with H/D exchange was firstly developed to determine and identify the volatile components in the fresh root and rhizome of Curcuma wenyujin. The TIC chromatograms of 3 batches of fresh roots harvested at different time showed significant difference in the volatile components:the constitution was the same but the content of them was different. More than 60 volatile components in fresh roots (Root of C. wenyujin) and rhizomes (Rhizome of C. wenyujin) of C. wenyujin were detected, of which 51 and 48 volatile components were identified respectively. The fresh roots and rhizomes of C. wenyujin were found to have the similar volatile components. The contents of these components were calibrated by the response of β‐elemene. In addition, the principal active component, β‐elemene, was further confirmed and relatively quantified by its standard. γ‐terpinene showed obvious allylic hydrogen/deuterium exchange using deuterium oxide which gave a new method to identify some compounds containing allylic hydrogen. At the same time, the active hydrogen compounds were also further confirmed. The results show that HS‐GC‐MS method is a fast, simple and efficient way for the analysis of volatile components from medical plants. |
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| Keywords: | Curcuma wenyujin static headspace GC-MS H/D exchange volatile component |
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