Determination of digitoxin in pharmaceutical oral dosages by automatic discrete-sample analysis

An automatic procedure for determination of digitoxin in pharmaceutical oral dosages is described. The procedure is based on the fluorimetric determination of digitoxin in hydrochloric acid media in the presence of hydrogen peroxide. Operational conditions and analytical setup are detailed. Results for repeatability, linearity, and tablet analysis are given. The procedure can be used for analysis of sets of up to 30 tablets at the rate of 17 readings per hour. Contents as low as 0.1 mg of digitoxin can be determined.