N.m.r. determination of wax esters in marine lipids |
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| Authors: | PJ Ke RG Ackman DL Hooper |
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| Institution: | Department of the Environment, Fisheries and Marine Service, Halifax Laboratory, 1707 Lower Water St., Halifax, Nova ScotiaCanada;Department of Chemistry, Dalhousie University, Halifax, Nova ScotiaCanada |
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| Abstract: | The proton n.m.r. spectra of lipids containing triglycerides and wax esters dissolved in CDCl3 are characterized by seven sets of signals. The areas of the signals of terminal methyl group and of methylene protons of both wax ester and triglyceride were integrated. These were used to calculate the content of wax esters in lipids or oils. The rapid n.m.r. procedure is directly usable for natural lipids containing as low as 3 mg of wax esters in 50-mg samples with an error of about 7%. The method described compares favorably with t.l.c. determination. |
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