| 芝麻致敏原的实时荧光PCR检测 |
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| 引用本文： | 蒋丹,郑秋月,赵昕,王刚,曹际娟.芝麻致敏原的实时荧光PCR检测[J].分子科学学报,2009,25(5). |
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| 作者姓名： | 蒋丹 郑秋月 赵昕 王刚 曹际娟 |
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| 作者单位： | 辽宁省出入境检验检疫局,辽宁,大连,116015 |
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| 基金项目： | 国家质量监督检验检疫总局公益性行业计划项目 |
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| 摘 要： | 针对芝麻Sesamum indicum 2S albumin mRNA基因设计引物、探针,在实时荧光PCR仪上进行扩增、检测和分析.结果显示:该组探针和引物对芝麻有很强的特异性,除黑芝麻和白芝麻外,其余6种对照材料均未检测到荧光信号,黑白芝麻成分检测灵敏度达到0.1%.该方法具有灵敏度高、快速、简便的特点,可用于芝麻致敏原成分的定量检测.
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| 关 键 词： | 芝麻 致敏原 实时荧光聚合酶链式反应 |
Detection of sesame allergen by real-time PCR |
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| JIANG Dan,ZHENG Qiu-yue,ZHAO Xin,WANG Gang,CAO Ji-juan.Detection of sesame allergen by real-time PCR[J].Journal of Molecular Science,2009,25(5). |
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| Authors: | JIANG Dan ZHENG Qiu-yue ZHAO Xin WANG Gang CAO Ji-juan |
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| Institution: | JIANG Dan,ZHENG Qiu-yue,ZHAO Xin,WANG Gang,CAO Ji-juan*(Liaoning Entry-Exit Inspection and Quarantine Bureau,Dalian 116015,China) |
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| Abstract: | The 2S albumin mRNA gene was selected to design the specific primers and probe for the detection of sesame by real-time PCR.The results show that the primers and probe are specific.No fluorescenc signal was detected in the other 6 control materials but sesamenut.The sensitivity of the detecting method could be 0.1%.The detecting method for sesamenut is sensitive,fast,and simple.And it can be applied for the quantitative detection of sesamenut allergen. |
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| Keywords: | sesame allergen real-time PCR |
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