Analysis of streptolydigin degradation and conversion in cultural supernatants of <Emphasis Type="Italic">Streptomyces lydicus</Emphasis> AS 4.2501 |
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Authors: | Liangzhi Li Jiaolong Fu Yexian Qiu Jinlian Wang |
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Institution: | (1) State Key Laboratory of Oncology in South China, Room 619, Cancer Center, Sun Yat-sen University, 651 Dongfeng Road East, Guangzhou, 510060, China;(2) Beijing Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100081, China;(3) Department of General Surgery, Chengdu Army General Hospital, Chengdu, 610083, China;(4) Department of Endocrinology, Chengdu Army General Hospital, Chengdu, 610083, China; |
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Abstract: | A variety of structural analogues of streptolydigin exists in the cultural supernatants of Streptomyces lydicus AS 4.2501. The degradation of streptolydigin in cultural supernatants with different pH values kept at 25°C in a thermostatic
bath was investigated using LC-MS/MS detection. Analysis of the alkaline supernatants (pH 9.50) provides evidence of degradation
and conversion between streptolydigin and its structural analogues. Interestingly, a new streptolydigin analogue was detected
by LC-MS and photo-diode array (PDA) detection in the process of alkaline degradation. After 48 h in a thermostatic bath,
the degradation of streptolydigin and its two analogues at pH 9.50 approached pseudo-first order kinetics. Comparatively,
the degradation of streptolydigin was much more rapid in the cultural supernatants with pH 3.05, only requiring 2 hours. Qualitative
analysis of the degradation products by LC-MS/MS and PDA indicated that hydrolysis of the epoxy ether bond and acid amide
bond was the major mechanism of degradation in acidic cultural supernatants. Two degradation products in the acid supernatant
were assumed. |
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