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Development of an amine dehydrogenase for synthesis of chiral amines |
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| Authors: | Abrahamson Michael J Vázquez-Figueroa Eduardo Woodall Nicholas B Moore Jeffrey C Bommarius Andreas S |
| Affiliation: | School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Petit Institute of Bioengineering and Bioscience, Atlanta, GA 30332, USA. |
| Abstract: | A leucine dehydrogenase has been successfully altered through several rounds of protein engineering to an enantioselective amine dehydrogenase. Instead of the wild-type α-keto acid, the new amine dehydrogenase now accepts the analogous ketone, methyl isobutyl ketone (MIBK), which corresponds to exchange of the carboxy group by a methyl group to produce chiral (R)-1,3-dimethylbutylamine. |
| Keywords: | amine dehydrogenase asymmetric synthesis biocatalysis chiral amines directed evolution |
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