Sol particle immunoassays using colloidal gold and neutron activation |
| |
| Authors: | R. Zeisler S. F. Stone R. P. Viscidi E. H. Cerny |
| |
| Affiliation: | (1) Center for Analytical Chemistry, National Institute of Standards and Technology, Gaithersburg, Maryland, (USA);(2) The Eudowood Division of Infectious Diseases, Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, (USA);(3) Fondation pour la Recherche Medicale, University of Geneva, 64 Avenue de la Roseraie, 1211 Geneva 4, (Switzerland);(4) Present address: Seibersdorf Laboratories, International Atomic Energy Agency, P.O. Box 100, A-1400 Vienna, Austria;(5) Present address: Department of Trace Elements in Health and Disease, Hahn Meitner Istitute, Glienicker Strasse 100, W-1000 Berlin 39, Germany |
| |
| Abstract: | The feasibility of performing immunoassays with colloidal gold labels and detection of198Au by neutron activation has been demonstrated with measurements of human immunoglobulin and of serum antibodies to human immunodeficiency virus type 1. The detection sensitivity achieved after activation in a high flux reactor or with a water moderated252Cf source, by gamma-counting or by autoradiography, is similar to the sensitivity obtained with absorbance measurements in the more common enzyme immunoassays. The reactor based neutron activation assay allows detection of 10–16 mol of analyte in routine operation with possible extension to 10–20 mol. The sensitivity with the 1.3 Ci252Cf source is limited to about 10–15 mol. The practical limitations of the assay's sensitivity at this point are due to background signals from reagents and/or nonspecific binding of the gold labeled reagent. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
