| 日本血吸虫卵壳蛋白编码基因的扩增及克隆 |
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| 引用本文： | 张莉,张雷,李伟.日本血吸虫卵壳蛋白编码基因的扩增及克隆[J].大理学院学报,2009,8(12):25-27. |
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| 作者姓名： | 张莉 张雷 李伟 |
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| 作者单位： | 大理学院基础医学院,云南,大理,671000 |
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| 基金项目： | 大理学院科研基金资助项目 |
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| 摘 要： | 目的:扩增和克隆日本血吸虫卵壳蛋白编码基因(SjESG),以研究其作为候选疫苗分子和药物靶点的可能性.方法:合成引物,以日本血吸虫雌、雄虫cDNA第一链为模板,RT-PCR扩增日本血吸虫卵壳蛋白编码基因,与带有硫氧还蛋白(Trx)基因的高效原核表达质粒pET32a(+)定向重组,构建原核表达重组质粒,并经限制性核酸内切酶酶切分析和PCR鉴定.结果:从雌虫cDNA中扩增出624bp SjESG基因,原核表达重组质粒pET32a(+)-SiESG经限制性核酸内切酶酶切和PCR均获得624bp的DNA片段.结论:成功构建原核表达重组质#ipET32a(+)-SjESG.
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| 关 键 词： | 日本血吸虫 卵壳蛋白基因 扩增 克隆 |
Amplification and Cloning of Eggshell Protein Gene of Schistosoma japonicum |
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| Zhang Li,Zhang Lei,Li Wei.Amplification and Cloning of Eggshell Protein Gene of Schistosoma japonicum[J].Journal of Dali University,2009,8(12):25-27. |
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| Authors: | Zhang Li Zhang Lei Li Wei |
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| Institution: | Zhang Li,Zhang Lei,Li Wei (College of Preclinical Medicine,Dali University,Dali,Yunnan 671000,China) |
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| Abstract: | Objective:To amplify and clone eggshell protein gene of Schistosoma japonicum,and explore the possibility of whether the eggshell protein might be used as potential targets for vaccine development. Methods:Primers were designed and synthesized. The DNA encoding eggshell protein of Schistosoma japonicum was amplified by using PCR with female SjDNA as template. The product from PCR was cloned into prokaryotic expressed vector pET32a (+) with thioredoxin (Trx) gene. The recombinant plasmid (pET32a (+)-SjESG) w... |
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| Keywords: | Schistosoma japonicum eggshell protein gene amplify clone |
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