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Elucidation of triacylglycerols in cod liver oil by liquid chromatography electrospray tandem ion-trap mass spectrometry |
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| Authors: | Ying-Xu Zeng Pedro Araujo Zhen-Yu Du Livar Frøyland |
| Institution: | a National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029, Nordnes, N-5817 Bergen, Norway b Department of Chemistry, University of Bergen, N-5009 Bergen, Norway c Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal |
| Abstract: | Though liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS2) has been widely used in the structural elucidation of triacylglycerols (TAG) in vegetable oils, its potentiality for the identification of TAG molecules in omega-3 rich oils remains unexplored till date. Hence, this article investigates the applicability of LC-ESI-MS2 for the structural characterization of naturally occurring TAG in cod liver oil without the TAG fractionation during the sample preparation. A computational algorithm was developed to automatically interpret the mass spectra and elucidate the TAG structures respectively. The results were compared against the lipase benchmark method. A principal component analysis study revealed that it is possible to discriminate genuine from adulterated cod liver oil. |
| Keywords: | Cod liver oil Triacylglycerols Fatty acids Liquid chromatography electrospray tandem mass spectrometry Algorithm |
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