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Label-free optical diagnosis of hepatitis B virus with genetically engineered fusion proteins |
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| Authors: | Shun Zheng Tae Jung Park Seok Jae Lee |
| Affiliation: | a Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea b BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea c NEMS-Bio Team, National Nanofab Center, 335 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea d Department of Bio & Brain Engineering, Department of Biological Sciences, and Bioinformatics Research Center, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea |
| Abstract: | A simple biosensing strategy for the diagnosis of patients with hepatitis B virus (HBV) was developed. This study can be divided into two themes, both of which utilized gold-binding polypeptide (GBP) fusion proteins: HBV surface antigen PreS2 (HBsAg) detection with GBP-fused single chain antibody (GBP-ScFv) and anti-HBsAg detection with GBP-HBsAg. These GBP-fusion proteins can directly bind onto the gold surface via the high binding affinity between the GBP and the gold surface, while at the same time, orient the recognition sites toward the sample for target binding. This one-step immobilization strategy, which greatly simplifies a fabrication process as well as maintaining biological activity of the recognition elements, can be applied to optical analytical methods, such as surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR). |
| Keywords: | Surface plasmon resonance Localized surface plasmon resonance Biosensor Hepatitis B virus Gold-binding polypeptide Fusion protein |
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