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Direct and rapid electrochemical immunosensing system based on a conducting polymer |
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| Authors: | B. Piro S. Reisberg L.A. Dang M.C. Pham |
| Affiliation: | a Laboratoire Interfaces-Traitements-Organisation et Dynamique des Systèmes (ITODYS), associé au CNRS-UMR 7086, Université Paris Diderot - Paris 7, 15 rue Jean-Antoine de Baïf, 75205 Paris Cedex 13, France b Université Paris 11, IFR 89, U-542 Inserm, Groupe Hospitalier Paul Brousse - 94800 Villejuif, France c Institute of Chemistry, VAST, 18 av. Hoang Quoc Viet, Cau Giay, Hanoi, Viet Nam |
| Abstract: | A system device using multifunctional conjugated copolymer poly(5-hydroxy-1,4-naphthoquinone-co-hydroxy-2-thioacetic acid-1,4-naphthoquinone) acting both as immobilizing and transducing element for reagentless immunosensor has been constructed. Its functionality was evaluated in an antigen-antibody interaction model using ovalbumin-anti-ovalbumin. It was shown that the system specifically detects via electrochemical signal the antigen-antibody immune interaction in a reagentless context. Comparison to the conventional ELISA technique relevant to performance and sensitivity was presented. |
| Keywords: | Conjugated polymer Electrochemical immunosensor Reagentless detection Antibody-antigen affinity ELISA assays |
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